Fig. 5

β3AR stimulation has no effect on tau phosphorylation in the hippocampus of 3xTg-AD mice. Phosphorylated and total tau protein measured in a–f detergent-soluble and h–k detergent-insoluble fractions of hippocampus homogenates of non-transgenic and 3xTg-AD mice. g Examples of Western blots. Homogenates were all run on the same gel, but consecutive bands were not taken for all representative photo examples and were cut-pasted to match the histogram order. Data are represented as mean ± SEM (n/group indicated in bars). Statistics: two-way ANOVA, effect of genotype: ^&&&&p < 0.0001 (a–k). Abbreviations: 3xTg-AD: triple transgenic mice; CL: CL-316,243-injected group; ins: insoluble; NonTg: non-transgenic mice; OD: optical density; S: saline-injected group; sol: soluble