Fig. 2

CL-316,243 treatment increases brown adipose tissue thermogenesis. a Graphical representation of body temperature recorded hourly by telemetric probe implanted in the intraperitoneal cavity during the first 2 weeks of experiment (before glucose tolerance and behavioral tests). Grey rectangles indicate the dark phase and black arrows point toward each i.p. injection of CL-316,243 or saline. b Mean amplitude of body temperature during the light (12-h, from 7 a.m.) and c the dark phase (12-h, from 7 p.m.). d Body temperature (T°) and e area under curve of the T° after the first CL-316,243 or saline i.p. injection. f Interscapular BAT weights sampled at sacrifice. Levels of g UCP1, h β3AR, and i mitochondrial oxidative phosphorylation system normalized on eEF2 proteins measured in BAT by Western Blot. j Examples of Western blots in BAT samples. Homogenates were all run on the same gel, but consecutive bands were not taken for all representative photo examples. Data are represented as mean ± SEM (n/group indicated in each column). Statistics: two-way ANOVA, effect of treatment: **p < 0.01; ***p < 0.001; ****p < 0.0001; effect of genotype: ^&p < 0.05; ^&&p < 0.01; ^&&&p < 0.001; ^&&&&p < 0.0001 (b, c, e–g, i); Tukey’s post hoc test: ⁺p < 0.05. Kruskal-Wallis (i), Dunn’s post hoc test: ^@p < 0.05 (h). Abbreviations: AUC: area under curve; 3xTg-AD: triple transgenic mice; β3AR: β3 adrenergic receptor; BAT: brown adipose tissue; CL: CL-316,243-injected group; CV, CIII, CIV, CII, CI: mitochondrial oxidative phosphorylation system complex V, III, IV, II, I; eEF2: eukaryotic elongation factor 2; NonTg: non-transgenic mice; OD: optical density; S: saline-injected group; T°: temperature; UCP1: uncoupling protein 1