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Table 1 Assay characteristics for each individual biomarker

From: Core Alzheimer’s disease cerebrospinal fluid biomarker assays are not affected by aspiration or gravity drip extraction methods

Analyte Amyloid β 1–40 Amyloid β 1–42 Total Tau Neurogranin Trunc P75 BACE-1 α-synuclein
Technology Sandwich ELISA (colorimetric)
Vendor Euroimmun Euroimmun Euroimmun Euroimmun Euroimmun Euroimmun
Product code EQ 6511–9601-L EQ 6521–9601-L EQ 6531–9601-L EQ 6551–       9601-L EQ 6541–9601-L EQ 6545–9601-L
Assay design Capture mAb ADx103 (2G3) ADx102 (21F12) ADx201 ADx451 (ADxNGCT1) ADx401  (5G7) ADx301
Detector mAb ADx101 (3D6) ADx101 (3D6) ADx215 ADx403 (ADxNGCI2) ADx402 (10B8F1) ADx302
Sample Incubation Sample volume (μL) 15 15 25 15 15 25
Detector volume (μL) 100 100 100 100 100 100
Incubation time (h) 4 4 4 4 4 4
Incubation temperature (°C) 18–25 18–25 18–25 18–25 18–25 18–25
Sample dilution factor (1:x) 21 No dilution No dilution No dilution No dilution No dilution
Run validation data %CV QC samples (n = 3; min-max) 9.6–14.5 4.5–20.8 9.5–10.2 3.6–6.4 5.9–12.4 4.3–5.8
%CV Kit controls (n = 2; min-max) 6.0–9.3 2.6–5.0 3,3–7.7 4.1–4.3 4.1–8.1 3.8–3.9
Intra-assay CV (%) (n = 2; min-max) 0.0–15.3 0.0–23.6 0.0–17.3 0.0–12.9 0.0–8.8 0.0–12.7
Calibrator Calibrator range (pg/mL) 54–986 81–1724 56–1552 53–1799 238–11,212 150–5988
Sample concentration range (pg/mL) p5 3785 188 251 154 1098 1226
p25 5933 383 342 229 1647 1742
p50 8500 589 405 350 2091 2311
p75 10,529 882 529 472 2904 2952
p95 13,542 1272 812 745 3583 4190
Minimum 2431 92 186 115 940 1085
Median 8501 589 405 349.5 2091.5 2311.5
Maximum 18,307 1592 922 1194 5172 5388
  1. Abbreviations: Aβ amyloid beta, mAb monoclonal antibody, p5 percentile 5, pg/mL picograms/milliltre, CV coefficient of variation, μL microlitre
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Alzheimer's Research & Therapy

ISSN: 1758-9193