Technology
|
Sandwich ELISA (colorimetric)
|
Vendor
|
Euroimmun
|
Euroimmun
|
Euroimmun
|
Euroimmun
|
Euroimmun
|
Euroimmun
|
Product code
|
EQ 6511–9601-L
|
EQ 6521–9601-L
|
EQ 6531–9601-L
|
EQ 6551– 9601-L
|
EQ 6541–9601-L
|
EQ 6545–9601-L
|
Assay design
|
Capture mAb
|
ADx103 (2G3)
|
ADx102 (21F12)
|
ADx201
|
ADx451 (ADxNGCT1)
|
ADx401 (5G7)
|
ADx301
|
Detector mAb
|
ADx101 (3D6)
|
ADx101 (3D6)
|
ADx215
|
ADx403 (ADxNGCI2)
|
ADx402 (10B8F1)
|
ADx302
|
Sample Incubation
|
Sample volume (μL)
|
15
|
15
|
25
|
15
|
15
|
25
|
Detector volume (μL)
|
100
|
100
|
100
|
100
|
100
|
100
|
Incubation time (h)
|
4
|
4
|
4
|
4
|
4
|
4
|
Incubation temperature (°C)
|
18–25
|
18–25
|
18–25
|
18–25
|
18–25
|
18–25
|
Sample dilution factor (1:x)
|
21
|
No dilution
|
No dilution
|
No dilution
|
No dilution
|
No dilution
|
Run validation data
|
%CV QC samples (n = 3; min-max)
|
9.6–14.5
|
4.5–20.8
|
9.5–10.2
|
3.6–6.4
|
5.9–12.4
|
4.3–5.8
|
%CV Kit controls (n = 2; min-max)
|
6.0–9.3
|
2.6–5.0
|
3,3–7.7
|
4.1–4.3
|
4.1–8.1
|
3.8–3.9
|
Intra-assay CV (%) (n = 2; min-max)
|
0.0–15.3
|
0.0–23.6
|
0.0–17.3
|
0.0–12.9
|
0.0–8.8
|
0.0–12.7
|
Calibrator
|
Calibrator range (pg/mL)
|
54–986
|
81–1724
|
56–1552
|
53–1799
|
238–11,212
|
150–5988
|
Sample concentration range (pg/mL)
|
p5
|
3785
|
188
|
251
|
154
|
1098
|
1226
|
p25
|
5933
|
383
|
342
|
229
|
1647
|
1742
|
p50
|
8500
|
589
|
405
|
350
|
2091
|
2311
|
p75
|
10,529
|
882
|
529
|
472
|
2904
|
2952
|
p95
|
13,542
|
1272
|
812
|
745
|
3583
|
4190
|
Minimum
|
2431
|
92
|
186
|
115
|
940
|
1085
|
Median
|
8501
|
589
|
405
|
349.5
|
2091.5
|
2311.5
|
Maximum
|
18,307
|
1592
|
922
|
1194
|
5172
|
5388
|