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Fig. 1 | Alzheimer's Research & Therapy

Fig. 1

From: Normal levels of KIF5 but reduced KLC1 levels in both Alzheimer disease and Alzheimer disease in Down syndrome: evidence suggesting defects in anterograde transport

Fig. 1

Validating antibodies used for KIF5 family members. a Western blotting was performed using the lysates of 2N adult mouse brain. Predicted molecular weights of mouse KIF5A, KIF5B, and KIF5C are 117, 110, and 109 kDa, respectively. Using normal rabbit IgG, we failed to detect specific signals at these molecular weights. b Lysates were prepared from the brain, kidney, and liver of 2N adult mice to compare the levels of KIF5 in tissues by Western blotting. c A plasmid encoding KIF5A-EGFP, EGFP-KIF5B, or GFP-KIF5C was transiently expressed in HEK293T cells for 36 h followed by cell lysis and Western blotting to evaluate the specificity of KIF5 antibodies. GFP was probed to indicate the presence of KIF5A-EGFP, EGFP-KIF5B, or GFP-KIF5C. β-Actin was employed as an internal control. Arrow indicates transfected KIF5s

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