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Table 9 Percent recovery of low-concentration and high-concentration plasma spiked with recombinant GFAP

From: Development of a novel, sensitive translational immunoassay to detect plasma glial fibrillary acidic protein (GFAP) after murine traumatic brain injury

Spike concentration (pg/mL)

%recovery* (mean ± SD)

 

All samples

Low-concentration samples (n = 2)

High-concentration samples (n = 3)

100

86.3 ± 25.8

67.1 ± 10.5

99.0 ± 25.8

1000

77.5 ± 11.8

73.1 ± 3.1

80.4 ± 15.6

10,000

79.7 ± 13.6

74.1 ± 0.6

83.5 ± 17.8

Mean

81.2 ± 17.3

71.4 ± 6.0

87.7 ± 19.5

  1. *%recovery = ((measured concentration spiked sample-measured concentration neat sample)/concentration of spike) × 100%. SD standard deviation