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Table 7 Dilution linearity of plasma spiked with recombinant GFAP protein

From: Development of a novel, sensitive translational immunoassay to detect plasma glial fibrillary acidic protein (GFAP) after murine traumatic brain injury

Dilution factor

Spike concentration (pg/mL)

Mean duplicate CV (%)

%recovery*

(mean ± SD) (n = 3)

1:1

2,000,000

11.5

37.6 ± 4.3

1:10

200,000

5.7

105.0 ± 4.9

1:100

20,000

9.8

116.4 ± 12.1

1:1000

2000

14.9

123.1 ± 40.9

1:10,000

200

3.9

114.5 ± 0.5

1:100,000

20

13.9

90.5 ± 15.0

1:1,000,000

2

38.1

369.2 ± 136.2

  1. *%recovery = (measured concentration adjusted for dilution/theoretical concentration) × 100%