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Table 7 Dilution linearity of plasma spiked with recombinant GFAP protein

From: Development of a novel, sensitive translational immunoassay to detect plasma glial fibrillary acidic protein (GFAP) after murine traumatic brain injury

Dilution factor Spike concentration (pg/mL) Mean duplicate CV (%) %recovery*
(mean ± SD) (n = 3)
1:1 2,000,000 11.5 37.6 ± 4.3
1:10 200,000 5.7 105.0 ± 4.9
1:100 20,000 9.8 116.4 ± 12.1
1:1000 2000 14.9 123.1 ± 40.9
1:10,000 200 3.9 114.5 ± 0.5
1:100,000 20 13.9 90.5 ± 15.0
1:1,000,000 2 38.1 369.2 ± 136.2
  1. *%recovery = (measured concentration adjusted for dilution/theoretical concentration) × 100%