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Table 3 Assay protocol modifications and summary of assay robustness results

From: Development of a novel, sensitive translational immunoassay to detect plasma glial fibrillary acidic protein (GFAP) after murine traumatic brain injury

Assay

Modification

Plasma pool

Replicate CV (%)

%recovery*

1

Standard protocol

(Capture 1 h,

Samples 2 h,

Detection 1 h)

Low

3.7

Intermediate

0.7

High

6.7

2

Capture 30 min

Low

6.2

76.8

Intermediate

3.7

89.4

High

7.4

94.8

3

Capture overnight

Low

9.6

90.7

Intermediate

7.1

115.5

High

3.9

108.7

4

Samples 1 h

Low

3.4

101.7

Intermediate

11.2

85.2

High

9.7

97.9

5

Samples overnight

Low

18.2

72.7

Intermediate

0.5

81.6

High

2.0

80.4

6

Detection 30 min

Low

2.4

100.1

Intermediate

2.3

110.8

High

0.4

118.4

  1. *%recovery = (concentration measured with modified assay protocol/concentration measured with standard assay protocol) × 100%. CV coefficient of variation