Assay | Modification | Plasma pool | Replicate CV (%) | %recovery* |
---|---|---|---|---|
1 | Standard protocol (Capture 1 h, Samples 2 h, Detection 1 h) | Low | 3.7 | – |
Intermediate | 0.7 | – | ||
High | 6.7 | – | ||
2 | Capture 30 min | Low | 6.2 | 76.8 |
Intermediate | 3.7 | 89.4 | ||
High | 7.4 | 94.8 | ||
3 | Capture overnight | Low | 9.6 | 90.7 |
Intermediate | 7.1 | 115.5 | ||
High | 3.9 | 108.7 | ||
4 | Samples 1 h | Low | 3.4 | 101.7 |
Intermediate | 11.2 | 85.2 | ||
High | 9.7 | 97.9 | ||
5 | Samples overnight | Low | 18.2 | 72.7 |
Intermediate | 0.5 | 81.6 | ||
High | 2.0 | 80.4 | ||
6 | Detection 30 min | Low | 2.4 | 100.1 |
Intermediate | 2.3 | 110.8 | ||
High | 0.4 | 118.4 |