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Table 3 Assay protocol modifications and summary of assay robustness results

From: Development of a novel, sensitive translational immunoassay to detect plasma glial fibrillary acidic protein (GFAP) after murine traumatic brain injury

Assay Modification Plasma pool Replicate CV (%) %recovery*
1 Standard protocol
(Capture 1 h,
Samples 2 h,
Detection 1 h)
Low 3.7
Intermediate 0.7
High 6.7
2 Capture 30 min Low 6.2 76.8
Intermediate 3.7 89.4
High 7.4 94.8
3 Capture overnight Low 9.6 90.7
Intermediate 7.1 115.5
High 3.9 108.7
4 Samples 1 h Low 3.4 101.7
Intermediate 11.2 85.2
High 9.7 97.9
5 Samples overnight Low 18.2 72.7
Intermediate 0.5 81.6
High 2.0 80.4
6 Detection 30 min Low 2.4 100.1
Intermediate 2.3 110.8
High 0.4 118.4
  1. *%recovery = (concentration measured with modified assay protocol/concentration measured with standard assay protocol) × 100%. CV coefficient of variation