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Table 1 Assay materials

From: Development of a novel, sensitive translational immunoassay to detect plasma glial fibrillary acidic protein (GFAP) after murine traumatic brain injury

Material Manufacturer Catalog number
MSD GOLD Small Spot Streptavidin SECTOR pate Meso Scale Discovery (Rockville MD) L45SA
GFAP antibody pair Abcam (Cambridge UK) Ab244094
Capture label (EZ-link NHS-biotin) Sigma (Burlington MA) 20217
Detection label (MSD gold sulfo-tag NHS-ester) Meso Scale Discovery (Rockville MD) R91A0 (150 nM)
Recombinant GFAP Abcam (Cambridge UK) Ab68428
Blocking buffer (5% BSA in PBS) Sigma (BSA) (Burlington MA) A7906
Assay diluent (1% BSA in PBS) Sigma (BSA) (Burlington MA) A7906
Wash (PBS-T) VWR (Tween) (Radnor PA) CAJTX251-7
Read buffer (MSD GOLD Read buffer A) Meso Scale Discovery (Rockville MD) R92TG
MESO QuickPlex SQ 120 Meso Scale Disocvery (Rockville MD) AI0AA-0