Table 1 Assay materials
Material | Manufacturer | Catalog number |
|---|---|---|
MSD GOLD Small Spot Streptavidin SECTOR pate | Meso Scale Discovery (Rockville MD) | L45SA |
GFAP antibody pair | Abcam (Cambridge UK) | Ab244094 |
Capture label (EZ-link NHS-biotin) | Sigma (Burlington MA) | 20217 |
Detection label (MSD gold sulfo-tag NHS-ester) | Meso Scale Discovery (Rockville MD) | R91A0 (150 nM) |
Recombinant GFAP | Abcam (Cambridge UK) | Ab68428 |
Blocking buffer (5% BSA in PBS) | Sigma (BSA) (Burlington MA) | A7906 |
Assay diluent (1% BSA in PBS) | Sigma (BSA) (Burlington MA) | A7906 |
Wash (PBS-T) | VWR (Tween) (Radnor PA) | CAJTX251-7 |
Read buffer (MSD GOLD Read buffer A) | Meso Scale Discovery (Rockville MD) | R92TG |
MESO QuickPlex SQ 120 | Meso Scale Disocvery (Rockville MD) | AI0AA-0 |