Skip to main content
Fig. 4 | Alzheimer's Research & Therapy

Fig. 4

From: Development of a novel, sensitive translational immunoassay to detect plasma glial fibrillary acidic protein (GFAP) after murine traumatic brain injury

Fig. 4

The assay shows high dilution linearity over a wide range of calibrator and plasma specimen dilutions. Assay calibrator was spiked into 3 individual plasma samples with very low endogenous GFAP to a concentration of 2,000,000 pg/mL. Serial dilutions from 10-fold to 1,000,000-fold were made and samples at each dilution step were assayed in duplicate. a Percent recovery of samples spiked with calibrator plotted against dilution factor. b ECL signal of samples spiked with calibrator were plotted against the dilution factor. Serial dilutions were performed from 2-fold to 64-fold on 3 plasma samples with high endogenous GFAP concentrations. c Percent recovery of diluted plasma samples plotted against dilution factor. d ECL signal of diluted plasma samples and calibrator plotted against dilution factor. Points represent mean concentration of duplicates and error bars represent ±SD. For some points, the error bars are shorter than the height of the symbol and therefore are not shown. AU: arbitrary units, ECL: electrochemiluminescence

Back to article page

Alzheimer's Research & Therapy

ISSN: 1758-9193