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Fig. 3 | Alzheimer's Research & Therapy

Fig. 3

From: Development of a novel, sensitive translational immunoassay to detect plasma glial fibrillary acidic protein (GFAP) after murine traumatic brain injury

Fig. 3

The assay has a wide quantitative dynamic range from 25 pg/mL to at least 16,533 pg/mL and a lower limit of detection of 9 pg/mL. Eight plasma samples with a very low or b very high expected GFAP concentrations were assayed in duplicate and c CV of duplicates were determined. d 16 blank replicates (1% BSA in PBS) were assayed to calculate an estimated lower limit of quantification and lower limit of detection as described in Table 6. a,b,d Mean concentration of replicates is plotted with each point representing an individual sample replicate and error bars representing ± SD. c Points represent mean sample concentration and CV of duplicates. ac Circles represent samples from female mice, squares represent samples from male mice. AU: arbitrary units, CV: coefficient of variation, SD: standard deviation, BSA: bovine serum albumin, PBS: phosphate-buffered saline, ECL: electrochemiluminescence

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