Skip to main content
Fig. 1 | Alzheimer's Research & Therapy

Fig. 1

From: Development of a novel, sensitive translational immunoassay to detect plasma glial fibrillary acidic protein (GFAP) after murine traumatic brain injury

Fig. 1

The assay is robust to changes in antibody incubation duration but less robust to changes in samples incubation duration. a Low-, b intermediate-, and c high-concentration plasma pools were assayed using the standard assay protocol (1 h capture antibody incubation, 2 h sample incubation, 1 h detection antibody incubation) and several protocols with modifications to incubation durations. Robustness to protocol modifications was assessed by observing the variation in sample duplicate measurements and the closeness of the measured sample concentrations under each protocol to the measurements under the standard protocol. Mean concentration of duplicates is plotted with each point representing an individual sample replicate and error bars representing ± SD. Differences in measured sample concentration compared to the standard assay protocol were analyzed by one-way ANOVA with Dunnett’s multiple comparisons test and displayed within the graphs as *p < 0.05. o/n: overnight, SD: standard deviation

Back to article page

Alzheimer's Research & Therapy

ISSN: 1758-9193