Fig. 3
From: CERTL reduces C16 ceramide, amyloid-β levels, and inflammation in a model of Alzheimer’s disease
AAV-mediated neuronal expression of CERTL in mouse brain. a The recombinant genomes of the two AAV-2 vectors. ➔ Abbreviations: From left to right, ITR, inverted terminal repeats; hSYN1, human synapsin 1 gene promoter; CERTL, cDNA sequence coding for ceramide transfer protein long isoform (hCERTL, 1875 bp NP_005704.1); IRES, internal ribosome sequence for translation initiation; EGFP, cDNA coding for enhanced green fluorescent protein (GFP); WPRE, woodchuck hepatitis virus posttranscriptional control element; bGH, bovine growth hormone gene-derived polyadenylation site; TB, synthetic transcription blocker. b Representative images of immunofluorescent staining of cortical brain area from 5xFAD animals treated with AAV-control or AAV-CERTL. Section was co-stain for CERTs protein (green), neuronal marker NeuN (red). Scale bar 200 μM and 50 μM. c Western blot showing band intensities of CERTs and GAPDH. d Relative quantification of CERTs levels normalized to GAPDH in cortical protein extract from WT and 5xFAD animals treated with AAV-control or AAV-CERTL. Bars represent mean one representative experiment with 5–7 samples per group (Mann–Whitney test, *p < 0.05)