Fig. 4From: RETRACTED ARTICLE: Preservation of dendritic spine morphology and postsynaptic signaling markers after treatment with solid lipid curcumin particles in the 5xFAD mouse model of Alzheimer’s amyloidosisSLCP treatment prevented abnormal dendritic arborization and loss of dendritic spines in the PFC and entorhinal cortex of 5xFAD mice. Six- and twelve-month-old 5xFAD and age-matched control mice were treated with SLCP (100 mg/kg) or vehicle for 2 months and then their brains were extracted and stained with Golgi-Cox stain over a 2-week period. Coronal sections (120 μm) were stained with 75% ammonium solution and 1% sodium thiosulphate. Cortical pyramidal neurons (layer II-III), along with dendritic spines from apical and basal branches (primary, secondary, and tertiary) were imaged using × 40 and × 100 objectives, respectively. a Representative images from layer II cortical pyramidal neurons processed with Golgi-Cox stain. Note that apical and basal branches are relatively less in vehicle-treated 5xFAD mice and that SLCP treatment prevented this loss. b, d Representative dendritic spine images from apical and basal branches. c, e Morphometric data revealed that the number of dendritic spines were significantly decreased in vehicle-treated 5xFAD mice in comparison to their WT counterparts, whereas SLCP treatment mitigated these losses. f Representative images of layer II pyramidal neurons from entorhinal cortex. Fewer apical and basal branches were observed less in the vehicle-treated 5xFAD mice, but SLCP treatments mitigated this loss. g, i Representative dendritic spine images from apical and basal branches. h, j Dendritic spine density was significantly decreased in 5xFAD mice in comparison to WT mice, but SLCP treatment prevented much of this loss. k Representative images of apical and basal dendrites. l Representative images of primary, secondary, and tertiary dendrites from apical branch. Scale bar = 100 μm and is applicable to all images. *p < 0.05, **p < 0.01 in comparison to WT + vehicle, 5xFAD + SLCP, and WT + SLCPBack to article page