Fig. 7
From: Metformin attenuates plaque-associated tau pathology and reduces amyloid-β burden in APP/PS1 mice
Metformin reduced accumulation of autophagy-related protein in microglia in APP/PS1 mice. a Representative images of p62+ aggregates (green) in the brains of APP/PS1mice. Scale bars, 50 μm. b Confocal images of Iba1+ microglia (red) and p62+ aggregates (green) in the brains of APP/PS1 mice. Scale bars, 50 μm. c Quantification of percent area covered by p62 staining in APP/PS1 mice. Data were analyzed by one-way ANOVA followed by the Bonferroni post hoc test. Values are presented as mean±SEM. n = 5 per group. d, e Quantitative analysis of p62+ aggregates in microglia, expressed as the percentage of Iba1+ area that is also p62+ (d) and the percentage of the number of Iba1+ microglia containing p62+ aggregates (e). Data were analyzed by one-way ANOVA followed by the Bonferroni post hoc test. Values are presented as mean±SEM. n = 5 per group. *P < 0.05 vs. ctr+veh group; #P < 0.05 vs. tau+veh group; &P < 0.05 vs. ctr+met group