Fig. 6
From: Metformin attenuates plaque-associated tau pathology and reduces amyloid-β burden in APP/PS1 mice
Immunofluorescence analysis of microglia in relation with Aβ plaques and NP tau pathologies in APP/PS1 mice. a Representative confocal images of Iba1+ microglia (green) surrounding 6E10+ Aβ plaques (red) in the brains of APP/PS1 mice. Scale bars, 100 μm. b Quantification of the number of microglial cells surrounding plaques in APP/PS1 mice. Data were analyzed by one-way ANOVA followed by the Bonferroni post hoc test. Values are presented as mean±SEM. n = 5 per group. *P < 0.05 vs. ctr+veh group; #P < 0.05 vs. tau+veh group; &P < 0.05 vs. ctr+met group. c Representative confocal images of Iba1+ microglia (green) and AT8+ NP tau (red) in the brains of APP/PS1 mice. Scale bars, 50 μm. d Percentage of AT8+ microglial cells with respect to total Iba1+ microglial cells in the in PS19BE-injected APP/PS1 mice treated with or without metformin. Data were analyzed by Student’s t test. Values are presented as mean±SEM. n = 5 per group. #P < 0.05 vs. tau+veh group