Fig. 3

Accuracy validation of QPLEX™ kit by using randomized sample selection. a Random groups were generated (sorted by age) and 10 groups were blind-tested according to our QPLEX™ algorithm. Three models were tested: model I, 70.6% accuracy; model II, 75.0% accuracy; model III, 81.5% accuracy. b The detailed algorithm process to validate our biomarker models by using randomized sample selection. Random groups were generated by randomizing analysis (create random groups using “age” with case identification). Three hundred participants were classified as 10 groups and validated using their predicted probabilities (P). Accuracy (correct number of participants/total number of participants, %) of each group were calculated and averaged