Fig. 2From: Conditional genetic deletion of CSF1 receptor in microglia ameliorates the physiopathology of Alzheimer’s diseaseThe deletion of CSF1R does not affect microglia survival and delay cognitive decline in APP cKO mice. a–b Representative image of microglia (Iba1 green) in APPSwe/PS1 (6–8 months old) and APP cKO (6–8 months old). Scale bar = 500 μm and 20 μm. c Unbiased stereological count of microglia in 6–8 months old APPSwe/PS1 and APP cKO. APP 6m, n = 6, APP 8m n = 4, n = 6 APP cKO 6m, and n = 4 APP cKO 8m. d NOR with 3-time points comparison of WT, APPSwe/PS1, and APP cKO from 3 to 8 months (###, ## Discrimination differs from 0.5, APPSwe/PS1 6-month-old ***p = 0.0002, 8-month-old ***p = 0.0007). n = 10 for all groups. e Average time of observation during NOR acquisition phase. f Nesting with 3-time points comparison of WT, APPSwe/PS1, and APP cKO from 3 to 8 months. Score range from 1 (untouched square) to 5 (perfect nest) (APPSwe/PS1 8-month-old ***p = 0.0009). n = 10 in all groups. g Western blot analysis on 6- and 8-month-old APP cKO and their littermate controls. APPSwe/PS1 and APP cKO n = 7 (SMI312 heavy chain, *p = 0.0352). h Unbiased count of microglia per plaque in the hippocampus and cortex. APPSwe/PS1 and APP cKO n = 6 (cortex APP cKO 6 months *p = 0.0311)Back to article page