Table 1 Antibodies used in IHC
Target | Antibody/antigen | Dilution | Source | Secondary antibody | Dilution | Source |
|---|---|---|---|---|---|---|
p-Tau | AT8 (pSer202, pThr205) | 1:200 | ThermoFisher | AlexaFluor555 (anti-mouse) | 1:200 | ThermoFisher |
Microglia | Iba1 | 1:100 | Abcam | AlexaFluor647 (anti-rabbit) | 1:200 | ThermoFisher |
Astrocytes | GFAP | 1:200 | Cell Signaling | AlexaFluor594 (anti-mouse) | 1:200 | ThermoFisher |
Neuronal Nuclei | NeuN | 1:100 | Cell Signaling | AlexaFluor647 (anti-rabbit) | 1:200 | ThermoFisher |
Postsynapse | Homer | 1:2000 | Synaptic System | AlexaFluor594 (anti-guinea pig) | 1:500 | Synaptic System |
Presynapse | Bassoon | 1:200 | Synaptic System | AlexaFluor647 (anti-rabbit) | 1:500 | ThermoFisher |