Fig. 9

CLR01 treatment reduces tau seeding of hippocampal brain extracts in tau-biosensor cells. The soluble or insoluble fraction of brain extracts from P301S-tau mice were added to tau-biosensor cells and incubated for 48 h. The cells were visualized by a fluorescence microscope and their integrated FRET density was measured by flow cytometry. a–d Representative micrographs of a control, unseeded biosensor cells. b Biosensor cells seeded with the soluble fraction of a mouse in the vehicle-treated group. c Biosensor cells seeded with the soluble fraction of a mouse in the low-dose group. d Biosensor cells seeded with the soluble fraction of a mouse in the high-dose group. e Integrated FRET density analysis of the soluble fraction. f Spearman correlation between grip-strength and integrated FRET density in the hippocampus. g Spearman correlation between grip-strength and integrated FRET density in the whole brain (calculated by adding the values measured for the different brain regions). h Integrated FRET density analysis of the insoluble fraction. The data in panels e and h are presented as mean ± SD. P values were calculated using a one-way ANOVA with post hoc Tukey test