Fig. 1
From: Exploring the role of RALYL in Alzheimer’s disease reserve by network-based approaches
Pipeline of the MPN to prioritize modules and genes directly associated with AD reserve in our study. Gray and yellow block: data download and organization. Blue block: MPN modeling. Gene module: applied WGCNA to GSE1297 microarray expression data to identify co-expression modules and calculate the correlation between modules and each phenotype. AD reserve-module network, modules, and phenotypes are combined using conditional independence relationships (via Bayesian networks) to identify direct relationships among gene modules, AD traits, and reserve. The module most strongly associated with reserve is the target module and independently validated in the ROSMAP expression data. AD reserve hub gene: exploring the AD reserve hub genes in the target module and trajectory inference. Green block: RALYL expression validated in multi-AD RNA-seq data