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Table 1 Kinetic analysis of K11 binding to different Aβ peptide variants

From: Targeting isoaspartate-modified Aβ rescues behavioral deficits in transgenic mice with Alzheimer’s disease-like pathology

Peptide

KD value [nM]

isoD7-Aβ(1–18)

6

N7-Aβ(1–18)

2560

Aβ(1–18)

2700

isoD7-Aβ(1–40)

4

Aβ(1–40)

1670

isod7-Aβ(1–17)

2690

mouse isoD7-Aβ(1–18)

153

isoD7-3NY10-Aβ(1–18)

652

isoD7-PhosphoSer8-Aβ(1–18)

No binding

pGlu3-isoD7-Aβ(3–18)

2

pGlu3-Aβ(3–18)

402

  1. Binding affinities of immobilized antibody K11 to different Aβ peptides were determined by using a Biacore 3000 at 25 °C. Goat anti mouse IgG was immobilized on a CM5 sensor chip, followed by binding of mouse anti isoD7-Aβ antibody K11. Kinetic constants were determined by applying Aβ peptides at different concentrations and calculated from the combined set of data by using BIAevaluation software (Biacore AB)
  2. isoD7 l-isoaspartate, isod7 d-isoaspartate, 3NY10 nitrotyrosin at position 10, PhosphoSer phosphoserine at position 8, pGlu3 pyroglutamate at position 3
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Alzheimer's Research & Therapy

ISSN: 1758-9193