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Fig. 5 | Alzheimer's Research & Therapy

Fig. 5

From: Targeting isoaspartate-modified Aβ rescues behavioral deficits in transgenic mice with Alzheimer’s disease-like pathology

Fig. 5

Quantification of total Aβ and isoD7-Aβ peptides in 5xFAD mice brain treated with K11_IgG2a, 3D6_IgG2a, and isotype control. Three-month-old 5xFAD mice were treated weekly by intraperitoneal injections with 12 mg/kg K11_IgG2a, 3D6_IgG2a, and isotype control. After 38 weeks of treatment, mice were sacrificed and the left hemisphere was homogenized in T-Per buffer, followed by centrifugation. a In the resulting supernatants (T-Per fractions), the amounts of total Aβ and isoD7-Aβ were analyzed by ELISA. b The pellet was dissolved in 5 M GdmCl, again centrifuged, and the supernatants applied to a total Aβ- or isoD7-Aβ-specific ELISA. For statistical analysis Tukey’s multiple comparison test was used. Sample size was at least 10 animals per group. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001. The error bars represent SEM

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