Fig. 4
Upregulation of p47phox in neurons and astrocytes of aged APP/PS1 mice brain. a Serial sections of WT and APP/PS1 mice aged 12 months were stained for p47phox (green fluorescence) and MAP 2 (red fluorescence) as described in the “Materials and methods” section. Nuclei were stained with DAPI (blue fluorescence). The scale bar in the upper left panel is 25 μm. Selected areas are enlarged by five times and shown as combined as well as individual fluorescence stains. Quantification of the fluorescence of p47phox (b) and the number of p47phox colocalized with MAP 2 (c) are shown. d Serial sections of WT and APP/PS1 mice aged 12 months were stained for p47phox (green fluorescence) and GFAP (red fluorescence) as described in the “Materials and methods” section. Nuclei were stained with DAPI (blue fluorescence). The scale bar in the upper left panel is 25 μm. Selected areas are enlarged by five times and shown as combined as well as individual fluorescence stains. e Quantification of the number of p47phox colocalized with GFAP is shown. Both male and female mice were used. Data are mean ± SEM, based on four viewing fields for each region, using at least two mice. *p < 0.05, ***p < 0.001, ****p < 0.0001 compared with WT mice