Fig. 2
p47phox deficiency does not affect Aβ levels in APP/PS1 mice. Representative Western blots showing Aβ deposits in the hippocampus and the cortex of WT, APP/PS1, and APP/PS1-Ncf1−/− mice aged 9 months (a) and 12 months (c). b, d Quantification of immunoreactivity of Western blots, normalized against GAPDH. One-way ANOVA: b, hippocampus F (2, 8) = 27.46 p = 0.0003, cortex F (2, 6) = 31.56 p = 0.0007; d, hippocampus F (2, 8) = 33.30 p = 0.0001, cortex F (2, 9) = 26.55 p = 0.0002. Both male and female mice were used. Data are mean ± SEM, with 4–5 mice in each group. **p < 0.01, ***p < 0.001 compared with WT mice. e Representative immunofluorescence images of Aβ deposits in the brain of APP/PS1 and APP/PS1-Ncf1−/− mice, aged 12 months. Scale bar, 500 μm. Quantification of the fluorescence intensity (f) and number (g) of Aβ deposits are shown. Both male and female mice were used. Data are mean ± SEM, with 4–5 mice in each group. h, i Soluble and insoluble Aβ1–40 and Aβ1–42 from the brain of 12-month-old mice were measured using ELISA. Both male and female mice were used. Data shown are the means ± SEM, with 4 mice in each group