Table 1 Antibodies

TREM2

Goat

–

AF1828

R&D Systems

3.84 μg/mL / 1:30

Stimulus/ICC

TREM2

Rabbit

–

EPR20243

Abcam

1:500

WB

SYK pY525/ pY526

Rabbit

–

MA5-14918

Thermo Fisher

1:200

WB

Total SYK

Mouse

–

4D10

CST

1:500

WB

Fibronectin

Rabbit

–

15613-1-AP

Proteintech

1:500

WB

GAPDH

Rabbit

–

G9545

Sigma

1:2000

WB

Tubulin

Mouse

–

T5168

Sigma

1:1000

WB

Rabbit IgG

Goat

IRDye® 800CW

926-32211

LI-COR

1:3000–1:5000

WB

Mouse IgG

Donkey

IRDye® 680RD

926-68072

LI-COR

1:3000–1:5000

WB

Calnexin

Rabbit

–

ab22595

Abcam

1:1500

ICC

TGN-46

Rabbit

–

ab50595

Abcam

1:100

ICC

LAMP1

Rabbit

–

9091

CST

1:200

ICC

RAB9

Rabbit

–

5118

CST

1:100

ICC

TUJ1

Mouse

–

801201

Biolegend

1:500

ICC

Goat IgG

Donkey

Alexa Fluor® 488

A11055

Thermo Fisher

1:1000

ICC

Rabbit IgG

Donkey

Alexa Fluor® 568

A10042

Thermo Fisher

1:500

ICC

Rabbit IgG

Donkey

Alexa Fluor® 647

A31573

Thermo Fisher

1:500

ICC

Mouse IgG

Donkey

Alexa Fluor® 647

A31571

Thermo Fisher

1:500

ICC

IL-6

Rat

–

14-7069-81

Thermo Fisher

4 μg/ml

ELISA – capture

IL-6

Rat

Biotinylated

13-7068-81

Thermo Fisher

2 μg/ml

ELISA – detection

sTREM2

Rabbit

–

ab209814

Abcam

8 μg/ml

ELISA – capture

sTREM2

Goat

Biotinylated

BAF1828

R&D Systems

1.5 μg/ml

ELISA – detection

CD11B

Mouse (IgG1κ)

APC

301309

Biolegend

1:25

FC

CD14

Mouse (IgG1)

PE

21620144

Immuno-tools

1:25

FC

CD45

Mouse (IgG1)

APC

21335014

Immuno-tools

1:25

FC

αVβ3

Mouse (IgG1κ)

APC

304415

Biolegend

1:50

FC

αVβ5

Mouse (IgG1)

PE

FAB2528P

R&D Systems

1:50

FC

Control

Mouse (IgG1κ)

APC

400119

Biolegend

1:50

FC

Control

Mouse (IgG1)

PE

21335014

Immuno-tools

1:25

FC

Control

Mouse (IgG1)

APC

21275516

Immuno-tools

1:25

FC

Control

Mouse (IgG1)

PE

IC002P

R&D Systems

1:50

FC