Fig. 2

Brain expression of Aβ42 WT and mutants results in unique amyloid deposition. Newborn B6C3F1 pups were bilaterally injected ICV with 4 μl rAAV1-BRI-Aβ42 (10¹³ vg/ml) (WT, E22G, E22Q/D23N, ΔE22, S8A, S8E, or S26C). After 6 months, mice were euthanized, and brains were extracted and processed. a Representative brain sections were stained with pan-Aβ antibody and counterstained with hematoxylin. Scale bar, 60 μm, 200 μm, 500 μm; n = 4–10. b The second hemibrain was sequentially extracted in 2% SDS followed by 70% FA, and Aβ levels were quantified using Aβ sandwich ELISA with C-terminal-specific mAb as capture and pan-Aβ mAb as detection. Each dot represents an individual mouse brain, n = 4–10