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Table 1 Primary antibodies used in this study

From: Prenatal to early postnatal neurotrophic treatment prevents Alzheimer-like behavior and pathology in mice

Antibody Specificity Species Type Dilution Source
43D Total tau M Mono- 0.5 μg/mL WB Produced in-house
PHF-1 P-tau (Ser396/404) M Mono- 1:200 WB
1:500 IF
[39]
AT-8 P-tau (Ser202/T205) M Mono- 1:1000 WB Thermo Fisher Scientific
R134d Total tau R Poly- 1:1000 WB Produced in-house
GAPDH GAPDH R Poly- 1:1000 WB Santa Cruz Biotechnology
p-APP Thr668 R Poly- 1:1000 WB Cell Signaling Technology
APP APP R Poly- 1:1000 WB Generated in our institute
Anti-Aβ Aβ/APP R Mono- 1:200 IF Cell Signaling Technology
GluR1 GluR1 R Mono- 1:1000 WB EMD Millipore
Synaptophysin Synaptophysin M Mono- 1:3000 WB Millipore
SMI 52 MAP2 M Mono- 1:4000 WB Sternberger Monoclonals Inc.
PSD95 PSD95 R Poly- 1: 1000 WB Cell Signaling Technology
NR1 NMDAR1 R Mono- 1:500 WB ABCAM
GluR 2/3 GluR2 + 3 R Mono- 1:2000 WB ABCAM
Synapsin-1 Synapsin 1 R Poly- 1:40000 WB Enzo Life Sciences
CREB CREB M Mono- 1:1000 WB Cell Signaling Technology
p-CREB Ser133 R Mono- 1:1000 WB Cell Signaling Technology
Iba-1 Iba-1 R Poly- 1:1000 WB Wako Pure Chemical Industries, Ltd.
SMI22 GFAP M Mono- 1:2000 WB
1:1000 IF
Sternberger Monoclonals Inc. (Lutherville, MD,)
  1. Iba-1 ionized calcium-binding adaptor molecule 1, GFAP glial fibrillary acidic protein, p-tau phosphorylated tau, APP amyloid precursor protein, amyloid-β, Poly- polyclonal, Mono- monoclonal, R rabbit, M mouse