Fig. 6

Prenatal to early postnatal treatment with P021 prevents postsynaptic deficit at 4 months of age in 3xTg-AD mice. a, b Representative Western blots of the hippocampus for PSD95, synapsin, synaptophysin, MAP2, and GAPDH in 4- and 22-month-old mice. c, d Densitometric quantification of the above proteins after normalization with GAPDH. At 4 months, 3xTg-Vh mice showed a marked decrease in levels of PSD95, synapsin, synaptophysin, and MAP2, while P021 prevented PSD95 deficit (a, c). No significant change was found in 22-month-old mice (b, d). Data were analyzed by two-way ANOVA followed by Tukey’s multiple comparisons test. Further intergroup comparisons were performed using unpaired, two-tailed t test. n = 3–4 animals/group. *P < 0.05, **P < 0.01, ***P < 0.001