Fig. 5

Prenatal to early postnatal treatment with P021 prevents dendritic deficits and increases CREB activity at 4 months and 22 months of age in 3xTg-AD mice. a, b Representative Western blots of the hippocampus for NR1, NR2A, NR2B, GluR1, GluR2&3, p-CREB, CREB, and GAPDH at 4 months and 22 months in P021- or vehicle-treated mice. c, d Densitometric quantification of the above proteins after normalization with GAPDH. 3xTg-Vh mice showed a marked decrease of GluR1, while P021 treatment increased the levels of NR1, NR2A, GuR1, and p-CREB significantly at 4 months of age. At 22 months, P021 elevated the levels of NR1, NR2A, and p-CREB in 3xTg-AD mice. d Data were analyzed by two-way ANOVA followed by Tukey’s multiple comparisons test. Further intergroup comparisons were performed using unpaired, two-tailed t test. n = 3–4 animals/group. *P < 0.05, **P < 0.01, ***P < 0.001