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Fig. 4 | Alzheimer's Research & Therapy

Fig. 4

From: Prenatal to early postnatal neurotrophic treatment prevents Alzheimer-like behavior and pathology in mice

Fig. 4

Prenatal to early postnatal treatment with P021 prevents Aβ pathology in 22-month-old 3xTg-AD mice. a Aβ immunofluorescence in the coronal brain sections of one half of the brain (a–h: the high magnification, a and e for the subiculum, b and f for the piriform cortex, c and g for the forebrain, d and h for the hippocampus). b Thioflavin-S staining in the subiculum region in 3xTg-AD mice (i and j: the high magnification for the subiculum). c Representative Western blots of the hippocampus developed with antibodies to p-APP or APP. Quantification of the Aβ-positive staining area in one half of the brain (6 sections per mouse, n = 4 mice). d Amyloid plaque area seen by immunofluorescence in one half of the brain. e Amyloid plaque area detected by thioflavin-S staining in the subiculum (6 sections per mouse, n = 4 mice). f Relative level of phosphorylation of APP at Thr 668 after normalization with GAPDH (n = 4). Data were analyzed by two-way ANOVA followed by Tukey’s multiple comparisons test. Further intergroup comparisons were performed using unpaired, two-tailed t tests. *P < 0.05, **P < 0.01

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