Fig. 4
From: Amyloid precursor protein glycosylation is altered in the brain of patients with Alzheimer’s disease
CTFα and CTFβ remain unaltered in the AD frontal cortex. a Representative western blots of human frontal cortex tissue from NDC (closed symbol; n= 7) and AD (open symbol; n= 7) subjects probed with a C-terminal antibody (of the full-length APP). Densitometric quantification of the b CTFβ species, C99 (closed arrowhead) and C88 (open arrowhead) (for characterization see Fig. 1d) or c CTFα (C83), is shown; using GAPDH as a loading control to ensure equivalent amounts of protein were loaded in each lane. The calculations were performed in duplicate. d Graph of the quotient obtained for each sample by dividing the CTFβ (C99) immunoreactivity with that for CTFα (C83), for which no statistically significant differences were evident (as was also the case for the CTF quotients not shown: C99/C89; C89/C83)