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Fig. 1 | Alzheimer's Research & Therapy

Fig. 1

From: γ-Secretase modulators exhibit selectivity for modulation of APP cleavage but inverse γ-secretase modulators do not

Fig. 1

iGSM shifts γ-secretase process of many substrates beside rC100sub, but GSMs do not. Recombinant substrates were incubated with CHO cell membrane in the presence of GSM or iGSM. Aβ and Aβ-like peptide was immunoprecipitated with Ab5 antibody and analyzed by mass spectrometry (IP-MS). a rNotch1sub based on mouse Notch1 with DMSO, 1 μM GSM1, 1 μM Compound 2, 5 μM CA, 100 μM fenofibrate, or 12.5 μM ZLL. b Putative mouse Notch1 transmembrane domain and main cleavage sites observed in this study with GSMs and iGSMs. Weight of arrows indicates peak intensity in the spectra. c–i rC100sub, rNOTCH1-4sub, rCD44sub and rVEGFR1sub with DMSO, 1 μM GSM1, 100 μM fenofibrate, or 12.5 μM ZLL. * Unidentified peak (larger spectra are shown in Additional file 1)

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Alzheimer's Research & Therapy

ISSN: 1758-9193