Fig. 7
NJK14047 attenuates neurotoxicity of LPS-stimulated microglial cell line rather than that of LPS-stimulated astrocyte cell line. a Scheme of the experiment. Microglial cell line BV2 and astrocyte cell line C8-D1A were seeded on 6-well plate at 5 × 105 cells/well. BV2 and C8-D1A were stimulated with 500 ng/ml LPS alone or after 2 h of pre-treatment with either 1 or 10 μM NJK14047. After 22 h of LPS stimulation, all media were changed to the fresh neurobasal medium where cells were incubated for 24 h. The conditioned medium was collected to treat primary neurons seeded on 48-well plate for cytotoxicity assay (b) or 24-well cover glass for TUNEL assay (c). b Viability of mouse primary neurons stimulated with BV2 and C8-D1A conditioned media. O.D. at 570 nm were normalized to the control group and shown as percentage of control mean ± SD (n = 3, Kruskal-Wallis test). c TUNEL assay for mouse primary neurons stimulated with BV2 and C8-D1A conditioned media. Apoptotic cells were quantified using four images in each cover glass. Data are shown as mean ± SEM (scale bar = 100 μm, n = 5, one-way ANOVA). #P < 0.05 vs. the control group; *P < 0.05 vs. the LPS group