Fig. 7
Uptake of 18F-GE180 tracer in mouse hippocampus at day 0, day 3, and day 30. One-hour microPET imaging was carried out using a novel TSPO radioligand, 18F-GE180, to visualize the microglia activation in mouse brain pre- and post-antibody immunization. The tracer uptake was quantitatively estimated as the percentage of injected dose per unit volume of hippocampus. a–e Time-activity curve (TAC) of hippocampal 18F-GE180 uptake in 4-mo-old mice with a single injection of 0.25 ml PBS (a) or 500 μg of antibody including 3A1-IgG1 (b), 07/1-IgG1 (c), 07/2a-IgG2a (d), or 07/2a-k (e). e–h TAC of hippocampal 18F-GE180 uptake in 16-mo-old mice with a single injection of 0.25 ml PBS (f) or 500 μg of antibody including 3A1-IgG1 (g), 07/1-IgG1 (h), 07/2a-IgG2a (i), or 07/2a-k IgG2a (j). n = 3–4 mice per treatment group per age. Two-way ANOVA with Bonferroni post hoc test was applied for statistical analysis