Fig. 1
Anti-pGlu-3 Aβ IgG2a isotype mAb shows significant cognitive improvement in 15-mo-old Tg mice. No effect was observed on locomotor activity in the APPSWE/PS1ΔE9 Tg mice, as measured by total distance traveled in the open field (OF) (a). Less distance was spent in the center of the OF by PBS-treated Tg versus Wt mice (p < 0.05), and 07/1 treatment showed a trend (p < 0.0836) to reverse this (b). Day by day analysis of the acquisition phase of the water T-maze (WTM) shows there were fewer (p < 0.05) correct responses to find the platform in PBS-treated Tg versus Wt mice on days 3–8 (c). 07/2-treated Tg mice displayed a significant increase (p < 0.05) in the percent of correct responses on days 3 and 7 compared to Tg PBS-treated mice (c). Day by day analysis of the reversal phase of the WTM (c) demonstrated a reduction (p < 0.05) in percent of correct responses on days 2–4 by the PBS-injected Tg vs. Wt mice, indicating that the Tg mice had impaired cognitive flexibility. A significant increase in correct responses was observed in Tg mice treated with 07/2a (p < 0.05) on days 2 and 3 (c). WTM results averaged across all days confirmed that Tg mice made significantly fewer (p < 0.05) correct responses to find the platform compared to Wt PBS-injected mice in both the acquisition and reversal phases, which appeared to be partially rescued following 07/2a (p < 0.05) treatment compared to PBS Tg mice (d, e). n = 11–13 per group. All data are expressed as the mean ± SEM. Fisher’s PLSD: a *p < 0.05 Wt PBS versus Tg 3A1, Tg 07/1, and Tg 07/2a at 10 and 20 min, p < 0.05 Wt PBS versus Tg 07/1, Tg 07/2a at 30 min, #p < 0.05 Wt PBS versus Tg PBS. b, d, and e *p < 0.05 versus Tg PBS, #p < 0.05 Wt PBS versus all groups. c *p < 0.05 Tg PBS versus 07/2a, #p < 0.05 Wt PBS versus Tg PBS