Fig. 4

Methazolamide, melatonin, and Trolox protect from Aβ-mediated ROS generation in SH-SY5Y. Following 24 h incubation with Aβ42 (0–25 μM) in the presence or absence of MTZ (300 μM), MEL (100 μM), and Trolox (300 μM), ROS-generated species were detected with CellROX (5 μM), and nuclei counterstained with Hoechst (1 μg/ml). Images depict CellRox fluorescence (red signal) and DAPI DNA counterstaining; bar, 25 μm. The graph on the right illustrates the quantitation of CellROX fluorescence values normalized to DAPI signal using ImageJ analysis software; data is represented as mean ± SEM. **p < 0.01 and ***p < 0.001