Fig. 12

Assessment of PI3K-mediated Nrf2 activation in SH-SY5Y and primary neurons by dot and Western blot analyses. SH-SY5Y cells and primary cortical neurons were treated with MTZ (300 μM) and MEL (100 μM) in the presence/absence of the PI3K inhibitor LY294002 and the GSK-3 inhibitor SB216763 (both at 10 μM concentration). This was followed by an evaluation of Nrf2 reactivity in nuclear extracts by dot and Western blot analysis following the protocols described in the “Material and methods” section. a SH-SY5Y cells. b Primary neurons. In both cases, left panels illustrate the dot blot images and right panels the Western blot detection