Fig. 1

| ZGM1 binds to the Aβ monomer and promotes its aggregation. a Western blot analysis of Aβ42 (50 μM) aggregation after incubation with a series of small molecule compounds (50 μM) for 24 h; DMSO is the solvent; CGR refers to Congo red, which has been reported to inhibit Aβ aggregation; the Aβ-specific antibody 6E10 was used. b Structural formula of ZGM1. c MST assays. ZGM1 can directly bind to Aβ. EC50 = 85.8 ± 18.2 μM, S.N. = 27.0. d Western blot analysis of Aβ42 (50 μM) aggregates incubated with different concentrations of ZGM1 at 37 °C for 24 h. Lane 9: control (CGR) with Congo red, which inhibits Aβ aggregation; lane 8: control (DMSO) with the same content of DMSO as the other samples but without ZGM1. Lanes 2–7: Aβ42 (50 μM) treated with 50 μM, 5 μM, 1 μM, 200 nM, 50 nM, and 5 nM ZGM1. e Western blot analysis of Aβ40 (50 μM) aggregates incubated with different concentrations of ZGM1 at 37 °C for 24 h. Lane 1: control treated with the same content of DMSO as the other samples but without ZGM1; lane 2–4: Aβ40 (50 μM) treated with 0.5 μM, 5 μM, and 50 mM ZGM1