Fig. 3

Association of APOE allele combinations with AD brain lipidome. MDMS-SL was performed to quantify 14 lipid classes and 216 molecular lipid species of inferior parietal lobule brain samples of APOEε2/c (N = 8), APOEε3/3 (N = 6), and APOEε4/c (N = 11) genotypes. a–c Volcano plots show all 216 molecular lipid species quantified by MDMS-SL. Each point represents log2 fold change and −log10 p value of a particular lipid species. Significantly affected species at p < 0.05 cutoff are colored in blue (decreased) or red (increased). d Heatmap of all lipid subspecies of the 14 lipid classes. Each row in the heatmap represents unique lipid subspecies, denoted by lipid class code; within a class, rows are ordered by molecular mass; each column represents a sample. Data is presented as a z-score where red values are above average and blue values are below average. e Bar plots of the sum of all species within a lipid class. Statistics is by one-away ANOVA (p values shown at the bottom of each graph), followed by Tukey post hoc test (significant differences shown on the graph: *< 0.05, **< 0.01, N.S. no significance)