Fig. 3
a Epitope mapping of immune sera was performed by alanine scanning competition ELISA. Two overlapped epitopes have been detected comprising amino acids 4–9 PRQEFE and amino acids 7–13 EFEVMED. Percent of inhibition of antibody binding to Tau2–18 peptide with mutated peptides (alanine substitution of each single amino acid) is shown in the table. b Binding avidity of anti-tau2–18 antibodies generated in T5x mice was determined by surface plasmon resonance (SPR). Monomeric and oligomeric forms of recombinant human tau (2N4R) were passed through antibodies immobilized on a Protein A-coated sensor chip