Fig. 1

Crenezumab recognizes Aβ oligomers from in vitro and in vivo sources. Pre-formed (m)onomericAβ₄₂, (o)ligomericAβ₄₂, or (a)ggregatedAβ₄₂ were run on native PAGE at 1000, 500, and 250 ng per lane to visualize Aβ₄₂ banding patterns (a). Note that aAβ was too large to enter the gel. Antibodies were incubated with pre-formed Aβ₄₂ oligomers overnight at 4 °C. To visualize nondenatured oligomers, immunoprecipitated (IP) eluates using were run on native PAGE. Crenezumab recognizes both low molecular weight oligomers between 20 and 50 kDa and high molecular weight (HMW) oligomers between 250 and 700 kDa (b). Anti-Aβ IPs from the soluble fraction of PS2APP mouse brain homogenates were run on native PAGE. Crenezumab recognizes HMW oligomers (c). 6E10 and 4G8 were used as detection antibodies on all blots