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Fig. 6 | Alzheimer's Research & Therapy

Fig. 6

From: Targeting soluble tumor necrosis factor as a potential intervention to lower risk for late-onset Alzheimer’s disease associated with obesity, metabolic syndrome, and type 2 diabetes

Fig. 6

solTNF blockade with XPro1595 reverts diet-induced pre-frontal cortex IRS1/Akt impairment. ab HFHC diet increases phosphorylated IRS1 (Ser 307) (n = 6, diet effect P = 0.0117). HFHC diet XPro 1595 interaction modulated phosphorylated Akt (Thr 308) (n = 6, diet effect P = 0.0180) in the prefrontal cortex. c, d Immunoblotting demonstrating the impact of HFHC diet in the hypothalamic phosphorylated IRS1 (Ser 307) (n = 6, diet effect P = 0.0245) and phosphorylated Akt (Thr 308) (n = 6, diet effect P = 0.0014). Immunoblots are representative of two independent experiments. Band intensity was calculated using Image Studio Lite, and values were normalized to intensity of β-act. Immunoblot images were cropped for comparison. Data were analyzed by two-way ANOVA followed by Tukey’s multiple comparisons in GraphPad Prism 6. Bar height indicates mean of samples; error bars indicate standard error of the mean (SEM). Lowercase letters indicate post hoc analysis. Means with different lowercase letters are significantly different from each other, P < 0.05

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