Fig. 2

LDN/OSU-0215111 dose and treatment start time selection. a Control mice were treated with LDN/OSU-0215111 at doses indicated for 7 days (n = 3/group). Doses of 10, 20, and 40 mg/kg were found to significantly upregulate gliosome EAAT2. We therefore selected a treatment dose of 10 mg/kg for use in rTg4510 mice. b Similar to transgenic EAAT2 overexpression, a single compound treatment upregulated EAAT2 in gliosomes in control mice (n = 4/group). Likewise, compound treatment in rTg4510 mice reduced EAAT2 expression c rTg4510 mice exhibited a significant decrease in synaptosomal PSD-95 expression; however, a single treatment of compound normalized PSD-95 expression comparable to controls (n = 4/group). d Behavioral deficits in the Y-maze were seen at 1 month of age in rTg4510 mice which further deteriorated at 2 months of age and plateaued at 3 months (n = 25 and 29, respectively). We therefore chose 2 months of age for treatment start. *P < 0.05, **P < 0.01, ***P < 0.001