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Table 1 List of antibodies

From: An experimental model of Braak’s pretangle proposal for the origin of Alzheimer’s disease: the role of locus coeruleus in early symptom development

Primary antibody

Company/product number

Primary antibody

Secondary antibody (fluorescence)

Secondary antibody (biotinylation amplification)

Host

Conc.

Company/product

Conc.

Company/product

Conc.

Iba-1

Wako/019-19741

Rabbit

1:1000

Invitrogen/A31572

1:500

β1 AR

Abcam/ab3442

Rabbit

1:400

Vector/BA 1000

1:500

NET

Invitrogen/MA5-24647

Mouse

1:500

Invitrogen/A31570

1:500

DBH

Millipore/MAB308

Mouse

1:500

Invitrogen/A31570

1:500

TPH

Sigma/T0678

Mouse

1:400

Invitrogen/A31570

1:500

Vector/BA 9200

1:500

NeuN

Millipore/MAB 377

Mouse

1:100

Invitrogen/A31570

1:500

HT7

Invitrogen/MN1000

Mouse

1:500

Invitrogen/A31570

1:500

Vector/BA 9200

1:1000

GFP

Thermo Fisher/A11122

Rabbit

1:200

Invitrogen/A27034

1:500

AT8

Thermo Fisher/MN1020

Mouse

1:200

Vector/BA 9200

1:1000

  1. Iba-1 ionized calcium-binding adaptor molecule 1, AR adrenoceptor, NET norepinephrine transporter, DBH dopamine beta-hydroxylase, TPH tryptophan hydroxylase, NeuN neuronal nuclei, GFP green fluorescence protein, HT7 human tau 7, AT8 anti-tau 8