Fig. 1

HtauE14 uptake in the LC and spread. a1–a3 Expression of htauE14 (green) in DBH (red) LC neurons in a rat. b Somatodendritic mislocalization of htauE14 in an LC neuron (arrows). c1 Spread of htauE14 to the midline brain stem in an 8-month-old rat (12 weeks post-infusion). c2 Enlargement of GFP cells in c1 (yellow box). d1–d3 HT7 (red) expression in the LC in a 19-month-old rat (7 months post-infusion). Arrows indicate GFP and HT7 co-expressed cells. e1–e2 HT7 staining in the LC in the same rat as in d. f1–f2 Midline HT7⁺ dorsal raphe neurons (DRN). g1–g3 Co-expression of GFP and TPH in cells (indicated by arrows) in the midline. Scale bar, 50 μm