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Fig. 5 | Alzheimer's Research & Therapy

Fig. 5

From: Upregulation of MIF as a defense mechanism and a biomarker of Alzheimer’s disease

Fig. 5

MIF expression overlaps but is not restricted in the vicinity microglia and astrocytes. Fixed brains were dehydrated in 30% sucrose solution and embedded in O.C.T. for cryosectioning at 20 μm thickness. a MIF was detected by polyclonal MIF antibody and Alexa 488-labeled secondary antibody, and active astrocytes were detected by monoclonal GFAP antibody and Alexa 594-labeled secondary antibody. Nuclei were stained with DAPI, and brain sections were observed under fluorescent microscopy. Arrows point to GFAP-positive cells. Arrowheads point to possible MIF expressing cells. Scale bar, 200 μm and 50 μm in inserts. b Neuritic plaques were detected by monoclonal 4G8 antibody and Alexa 488-labeled secondary antibody, and microglia were detected by polyclonal Iba-1 antibody and Alexa 594-labeled secondary antibody. Nuclei were stained with DAPI, and brain sections were observed under fluorescent microscopy. Arrowheads point to possible MIF expressing cells. Scale bar, 50 μm

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