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Fig. 2 | Alzheimer's Research & Therapy

Fig. 2

From: Upregulation of MIF as a defense mechanism and a biomarker of Alzheimer’s disease

Fig. 2

Increased MIF secretion protects neuronal cells from Aβ-induced neurotoxicity. Cells were seeded on seeded onto 96-well plates and cultured 24 h prior to treatment. Aβ treatment was achieved by adding medium diluted Aβ1-42 oligomer stock solution (100 μM in sterile PBS) at the final concentration of 10 μM or 50 μM. LPS at the concentration of 100 ng/mL was used as a positive control for MIF secretion. Sixteen hours after treatment, the culture medium was collected and centrifuged prior to analysis. Media collected from RAW 264.7 (a), BV-2 (b), and SHSY-5Y (c) cell lines were measured for MIF concentrations by ELISA. Values represent mean ± SEM, n = 4. *p < 0.05 relative to controls by one-way ANOVA with Newman-Keuls post hoc tests. #p < 0.05 relative to LPS by one-way ANOVA with Newman-Keuls post hoc tests. d The same batch of media from SHSY-5Y cells were subjected to LDH assay to evaluate the cell membrane integrity. Values represent mean ± SEM, n = 4. *p < 0.05 relative to controls by one-way ANOVA with Newman-Keuls post hoc tests. e SH-SY5Y and SYMS cell lines were subjected to Aβ oligomer treatment at the final concentration of 50 μM. After 24-h treatment, cell viability was assessed by MTS assays. Values represent mean ± SEM, n = 3. *p < 0.05 relative to controls by two-way ANOVA with Bonferroni’s multiple comparison test

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