| | Aβ40 | Aβ42 | Aβ40: Aβ42 |
|---|
(pg/mg total protein) | (pg/mg total protein) |
|---|
WT | TG | WT | TG | TG |
|---|
3 months | 3.7 ± 0.9 | 44.3 ± 3.5 | 1.6 ± 0.4 | 14.8 ± 2.8 | 3.2 ± 0.3 |
6 months | 2.6 ± 0.6 | 90.0 ± 12.5 | 0.8 ± 0.1 | 55.4 ± 13.2 | 1.9 ± 0.2**, ## |
12 months | 4.2 ± 1.5 | 306.2 ± 38.5 | 1.7 ± 0.6 | 197.0 ± 26.3 | 1.6 ± 0.1*, ### |
20 months | 2.8 ± 1.2 | 1031.2 ± 184.4*** | 1.1 ± 0.4 | 340.6 ± 81.6*** | 3.4 ± 0.4 |
- Soluble amyloid-β (Aβ) was measured in the S1 supernatant fraction from neocortical homogenates of aging wild-type (WT) and APPswe/PS1dE9 transgenic (TG) mice, by using Meso Scale Discovery. Aβ40/42 values were normalized to total protein content and reported as the mean ± SEM of 3–4 independent experiments. Age-dependent increases in the concentration of soluble Aβ40/42 were observed exclusively in APPswe/PS1de9 mice. The proportion of Aβ40 in the Aβ40:Aβ42 ratio increased in 20- vs. 6- and 12-month-old TG mice. *p < 0.05, **p < 0.01, and ***p < 0.001 vs. 3-month-old TG mice, two-way ANOVA followed by Bonferroni posttests; ##p < 0.01 and ###p < 0.001 vs. 20-month-old TG mice, one-way ANOVA followed by Bonferroni posttests
