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Table 3 Increased levels of soluble Aβ40/42 in the neocortex of APPswe/PS1dE9 mice

From: Neuroinflammation and amyloid-beta 40 are associated with reduced serotonin transporter (SERT) activity in a transgenic model of familial Alzheimer’s disease

 40 42 40:42
(pg/mg total protein) (pg/mg total protein)
WT TG WT TG TG
3 months 3.7 ± 0.9 44.3 ± 3.5 1.6 ± 0.4 14.8 ± 2.8 3.2 ± 0.3
6 months 2.6 ± 0.6 90.0 ± 12.5 0.8 ± 0.1 55.4 ± 13.2 1.9 ± 0.2**, ##
12 months 4.2 ± 1.5 306.2 ± 38.5 1.7 ± 0.6 197.0 ± 26.3 1.6 ± 0.1*, ###
20 months 2.8 ± 1.2 1031.2 ± 184.4*** 1.1 ± 0.4 340.6 ± 81.6*** 3.4 ± 0.4
  1. Soluble amyloid-β (Aβ) was measured in the S1 supernatant fraction from neocortical homogenates of aging wild-type (WT) and APPswe/PS1dE9 transgenic (TG) mice, by using Meso Scale Discovery. Aβ40/42 values were normalized to total protein content and reported as the mean ± SEM of 3–4 independent experiments. Age-dependent increases in the concentration of soluble Aβ40/42 were observed exclusively in APPswe/PS1de9 mice. The proportion of Aβ40 in the Aβ40:Aβ42 ratio increased in 20- vs. 6- and 12-month-old TG mice. *p < 0.05, **p < 0.01, and ***p < 0.001 vs. 3-month-old TG mice, two-way ANOVA followed by Bonferroni posttests; ##p < 0.01 and ###p < 0.001 vs. 20-month-old TG mice, one-way ANOVA followed by Bonferroni posttests