| | IL-1β | IL-6 | TNF |
|---|
(pg/mg total protein) | (pg/mg total protein) | (pg/mg total protein) |
|---|
WT | TG | WT | TG | WT | TG |
|---|
3 months | 0.17 ± 0.01 | 0.14 ± 0.02## | 1.59 ± 0.71 | 2.08 ± 0.49 | 0.08 ± 0.03 | 0.09 ± 0.02 |
6 months | 0.18 ± 0.03 | 0.23 ± 0.02# | 2.80 ± 0.58 | 3.49 ± 0.33 | 0.11 ± 0.03 | 0.19 ± 0.03 |
12 months | 0.18 ± 0.02 | 0.33 ± 0.05** | 2.92 ± 0.45 | 3.20 ± 0.50 | 0.14 ± 0.02 | 0.18 ± 0.01 |
20 months | 0.27 ± 0.03 | 0.38 ± 0.04 | 4.39 ± 0.80 | 3.10 ± 0.54 | 0.23 ± 0.02 | 0.20 ± 0.02 |
- The concentration of IL-1β, IL-6, and TNF was measured in the S1 supernatant fraction from neocortical homogenates of aging wild-type (WT) and APPswe/PS1dE9 transgenic (TG) mice, by using Meso Scale Discovery. Genotype-induced effects on cytokine levels were solely observed for IL-1β, which was increased over age-matched control values at the 12-month time-point. The concentration of all pro-inflammatory cytokines increased with age, in both TG and WT animals. Values of IL-1β, IL-6, and TNF were normalized to total protein content and reported as the mean ± SEM of 3–4 independent experiments. **p < 0.01 vs. 12-month-old WT; #p < 0.05, ##p < 0.01 vs. 20-month-old TG, two-way ANOVA followed by Bonferroni posttests
