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Table 2 Increased levels of pro-inflammatory cytokines in the neocortex of wild-type and APPswe/PS1dE9 mice

From: Neuroinflammation and amyloid-beta 40 are associated with reduced serotonin transporter (SERT) activity in a transgenic model of familial Alzheimer’s disease

  IL-1β IL-6 TNF
(pg/mg total protein) (pg/mg total protein) (pg/mg total protein)
WT TG WT TG WT TG
3 months 0.17 ± 0.01 0.14 ± 0.02## 1.59 ± 0.71 2.08 ± 0.49 0.08 ± 0.03 0.09 ± 0.02
6 months 0.18 ± 0.03 0.23 ± 0.02# 2.80 ± 0.58 3.49 ± 0.33 0.11 ± 0.03 0.19 ± 0.03
12 months 0.18 ± 0.02 0.33 ± 0.05** 2.92 ± 0.45 3.20 ± 0.50 0.14 ± 0.02 0.18 ± 0.01
20 months 0.27 ± 0.03 0.38 ± 0.04 4.39 ± 0.80 3.10 ± 0.54 0.23 ± 0.02 0.20 ± 0.02
  1. The concentration of IL-1β, IL-6, and TNF was measured in the S1 supernatant fraction from neocortical homogenates of aging wild-type (WT) and APPswe/PS1dE9 transgenic (TG) mice, by using Meso Scale Discovery. Genotype-induced effects on cytokine levels were solely observed for IL-1β, which was increased over age-matched control values at the 12-month time-point. The concentration of all pro-inflammatory cytokines increased with age, in both TG and WT animals. Values of IL-1β, IL-6, and TNF were normalized to total protein content and reported as the mean ± SEM of 3–4 independent experiments. **p < 0.01 vs. 12-month-old WT; #p < 0.05, ##p < 0.01 vs. 20-month-old TG, two-way ANOVA followed by Bonferroni posttests