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Table 1 Saturation analysis of [3H]5-HT uptake in brain membrane preparations from wild-type and APPswe/PS1dE9 mice

From: Neuroinflammation and amyloid-beta 40 are associated with reduced serotonin transporter (SERT) activity in a transgenic model of familial Alzheimer’s disease

  V max K m Vmax/Km
(fmol/min/mg total protein) (μM) (μL/min/mg total protein)
WT TG WT TG WT TG
3 months 958 ± 55 1166 ± 88 0.076 ± 0.022 0.069 ± 0.029 12.6 ± 2.5 16.8 ± 3.1
6 months 1060 ± 76 1134 ± 72 0.078 ± 0.028 0.086 ± 0.027 13.6 ± 2.7 13.1 ± 2.6
12 months 1112 ± 89 1106 ± 83 0.094 ± 0.036 0.131 ± 0.043 11.8 ± 2.5 8.4 ± 2.0
20 months 1182 ± 93 792 ± 74* 0.099 ± 0.037 0.114 ± 0.048 11.9 ± 2.5 7.0 ± 1.6
  1. Values are reported as the mean ± SEM of 3–4 independent experiments, conducted on freshly prepared membranes from the neocortex of aging wild-type (WT) and APPswe/PS1dE9 littermate transgenic (TG) mice. The apparent maximal velocity rate (Vmax) for the uptake of [3H]5-HT was reduced in 20-month-old TG vs. WT animals, without a change in the apparent Michaelis constant (Km). By one-way ANOVA, there was a modest, age-dependent reduction in the efficiency (Vmax/Km) of SERT in APPswe/PS1dE9 mice, which did not reach between-group significance by Bonferroni post hoc tests. *p < 0.05 vs. age-matched WT, two-way ANOVA followed by Bonferroni posttests