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Table 1 Saturation analysis of [3H]5-HT uptake in brain membrane preparations from wild-type and APPswe/PS1dE9 mice

From: Neuroinflammation and amyloid-beta 40 are associated with reduced serotonin transporter (SERT) activity in a transgenic model of familial Alzheimer’s disease

 

V max

K m

Vmax/Km

(fmol/min/mg total protein)

(μM)

(μL/min/mg total protein)

WT

TG

WT

TG

WT

TG

3 months

958 ± 55

1166 ± 88

0.076 ± 0.022

0.069 ± 0.029

12.6 ± 2.5

16.8 ± 3.1

6 months

1060 ± 76

1134 ± 72

0.078 ± 0.028

0.086 ± 0.027

13.6 ± 2.7

13.1 ± 2.6

12 months

1112 ± 89

1106 ± 83

0.094 ± 0.036

0.131 ± 0.043

11.8 ± 2.5

8.4 ± 2.0

20 months

1182 ± 93

792 ± 74*

0.099 ± 0.037

0.114 ± 0.048

11.9 ± 2.5

7.0 ± 1.6

  1. Values are reported as the mean ± SEM of 3–4 independent experiments, conducted on freshly prepared membranes from the neocortex of aging wild-type (WT) and APPswe/PS1dE9 littermate transgenic (TG) mice. The apparent maximal velocity rate (Vmax) for the uptake of [3H]5-HT was reduced in 20-month-old TG vs. WT animals, without a change in the apparent Michaelis constant (Km). By one-way ANOVA, there was a modest, age-dependent reduction in the efficiency (Vmax/Km) of SERT in APPswe/PS1dE9 mice, which did not reach between-group significance by Bonferroni post hoc tests. *p < 0.05 vs. age-matched WT, two-way ANOVA followed by Bonferroni posttests